.~ Via microinjection of a nucleus into an embryo, egg cell, or embryonic cell
DEFINITION
Classification: 800/24
Via microinjection of a nucleus into an embryo, egg cell, or embryonic cell:
(under subclass 21) Method wherein the nucleic acid is contained within an intact nucleus, and said nucleus is microinjected into an isolated cell which is an egg cell or embryonic cell (e.g., embryonic stem cell, etc.) or a cell which is part of an embryo, and which cell is, or becomes, the germ line cell.
(1) Note. The original nucleus of the microinjected cell may or may not remain.
(2) Note. "Microinjection" involves injection into the cell through a very small diameter tube, needle, or micropipet.
